DIOSGENINA, PRINCIPIUL ACTIV AL EXTRACTELOR DE TRIGONELLA SP. AR PUTEA INDUCE APOPTOZA CELULELOR CANCEROASE MCF7 PRIN ACTIVAREA CASPAZELOR
DIOSGENIN, THE ACTIVE PRINCIPLE OF TRIGONELLA SP. EXTRACTS MAY INDUCE APOPTOSIS ON MCF7 CANCER CELLS THROUGH CASPASE ACTIVATION
George-Ciprian PRIBAC, Aurel ARDELEAN
“Vasile Goldis” Western University, Arad,
Faculty of Medicine, Pharmacy and Dental Medicine,
Department of Cell and Molecular Biology, no. 1 Feleacului St., gpribac@uvvg.ro
Abstract
Recent studies suggest that fenugreek and its active constituents may possess anticarcinogenic potential. The methods used were those of culture and measurement of MCF7 cell line growth using MTT methods. Apoptosis was detected using agarose gel electrophoresis. The ratio of apoptotic cell was measured using APO-BRDU kit. The distribution of cell cycle and of mithocondrial membrane potential was investigated by flow-cytometry. Caspase activity was evaluated using caspase-induced apoptosis detection kit. Western blot analysis was used to evaluate the level of mithochondrial Bcl-2 expression. The results observed were those of MCF7 cells growth inhibition due to diosgenin. However, the precise mechanism of diosgenin-induced apoptosis is still unclear. Concluding, diosgenin, obtained from Trigonela foenum-graecum extract may induce apoptosis of MCF7 cancer cells by caspase pathway activation.
Keywords: diosgenin, MCF7 cells, apoptosis, Trigonella sp.
Cuvinte cheie: diosgenina, celule MCF7, apoptoza, Trigonella sp.